Ribonucleoprotein purification and characterization using RNA Mango
نویسندگان
چکیده
منابع مشابه
RNA affinity tags for purification of RNAs and ribonucleoprotein complexes.
Intrinsic affinity tags are useful tools for the study of macromolecular targets. Although polypeptide affinity tags are routinely used in purification and detection of protein complexes, there has been a relative lack of powerful RNA affinity tags that can be embedded within RNA sequences. Here, the preparation and use of two RNA affinity tags against Sephadex or streptavidin are described. Th...
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ژورنال
عنوان ژورنال: RNA
سال: 2017
ISSN: 1355-8382,1469-9001
DOI: 10.1261/rna.062166.117